Applied Biosystems ABI 7500 Real-Time PCR System

Applied Biosystems ABI 7500 Real-Time PCR System

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Description

Includes:

  • One ABI 7500 RT-PCR system complete with all accessories

The ABI 7500 Real-Time PCR System is a powerful platform designed for laboratories requiring superior performance and maximum dye versatility. This third-generation system features an innovative optical system that enhances sensitivity and enables access to a broader range of fluorophores. Its variable excitation capability allows greater sensitivity for longer-wavelength (red) dyes.


Features & Benefits

  • Powerful, five-color platform:
  • Calibrated for a broad range of dyes, including FAM™/SYBR™ Green I, VIC™/JOE™, NED™/TAMRA™/Cy3™, ROX™/Texas Red™, and Cy5™.
  • Specialized optical system:
  • Allows easy and accurate calibration to new dyes without requiring additional filter sets.
  • Advanced multi-componenting algorithm:
  • Minimizes spectral crosstalk, making it ideal for multiplexing applications.
  • User-friendly software:
  • Includes plate setup wizards, multi-plate data viewing, and advanced analysis tools for simple and efficient data processing.


Specifications

ConditionNew
Stock Number25
Calibrated DyeSYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™
IncludesNotebook Computer
Reaction SpeedStandard
External ComputerNotebook: Intel Core 2 Duo T5500 (1.66 GHz), 8X DVD, 80 GB HD, 1.0 G DDR2-533 MHz SDRAM
For Use With (Equipment)7500 System
Max. Power950 W
Passive Reference DyeROX (Separate Tube), No ROX, ROX (Pre-mixed)
Precision99.7% confidence (5000 & 10000 copies)
Sample Ramp Rate± 1.1°C⁄sec
Software CompatibilityPrimer Express, 21 CFR Part 11 Module (optional), 7500 System Software (RQ included)
Temperature Range (Metric)4-100°C
Thermal Cycling SystemPeltier-Based System
DimensionsWidth: 34 cm (13.39 in), Depth: 45 cm (17.72 in), Height: 49 cm (19.29 in)
Voltage120V
Content And StorageAmbient
Max. Heating Rate (Thermal Block)2.5°C
Dynamic Range9 logs
Format96-well plate
High-throughput CompatibilityMultiplexing
OpticsCCD Camera, 5 excitation filters, Tungsten-halogen lamp, 5 emission filters
Peak Block Ramp Rate2.5°C⁄sec
Reaction Volume Range20-100 µl
Sensitivity1 copy of RNase P gene (human genomic DNA)
Temperature Accuracy0.25°C (35°C to 95°C after 3 min)
Temperature Uniformity0.50°C (after 30 sec)
Throughput4 to 5-96-well plates per 8-hr day
Weight75 lbs