Roche LightCycler 480 || Real Time PCR Benchtop System

Roche LightCycler 480 || Real Time PCR Benchtop System
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Description
Roche LightCycler 480 || Real Time PCR Benchtop System
Includes: One Roche Light Cycler and Accessories.
The LightCycler® 480 System is a proven high-performance, medium-to high-throughput PCR platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. Benefit from cutting-edge thermal block and optical technology that leads to excellent well-to-well homogeneity and maximized detection sensitivity. Generate meaningful results via the state-of-the-art data analysis methods, in line with MIQE guidelines, and analyze true raw data without the need for passive reference dyes or normalization plates.
The ideal combination of speed, accuracy and versatility
The LightCycler® 480 System features a versatile, plate-based, real-time PCR device that supports mono- or multicolor applications. The benchtop instrument is easily customizable to meet changing user requirements, and can be integrated into everyday use as an automated high-throughput solution.
Features/Benefits
Features:
Rely on the LightCycler® 480 System to perform:
Gene Quantification
Gene detection
Absolute quantification
Relative quantification/gene expression analysis
Genetic Variation Analysis
Melt-curve genotyping with HybProbe- or SimpleProbe probes
Endpoint genotyping with hydrolysis probes
Gene Scanning or Methylation analysis with High Resolution Melting (HRM)
Multiple Plate Analysis
96-or 384-well plates
Fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids, mutation scanning, and SNP analysis
Basic and advanced methods for gene expression and genotyping based on endpoint analysis or melting curves
Wide range of probe formats and fluorescent dyes
White or clear multiwell plates to best fit your assay format, application, and workflow
Test Menu
Gastro Parasite Panel | Gastro Virus Panel | Gastro Bacteria Panel | Respiratory Influenza Panel |
Giardia2 | Novovirus GG12 | Aeromonas2 | Influenza A2 |
Dientamoeba fragilis2 | Novovirus GG22 | Yersinia enterocolitica2 | Influenza B2 |
Cryptosporidium spp2 | Sapovirus1,2 | Campylobacter2 | Influenza A H1 (H1N1)1,2 |
Blastocystis2 | Rotavirus2 | Shigella/EIEC2 | Influenza A H31 |
Entamoeba histolitica2 | Adenovirus1,2 | Salmonella2 | Influenza A H51 |
Emerging Pathogens Panel | Astrovirus1,2 | Plesiomonas1 | Influenza A H7 (H7N9)1 |
Zika Virus2 | Enterovirus1 | Influenza A H91 | |
Respiratory Virus Panel | Respiratory Coronavirus Panel | Carbapenemase Panel | Respiratory Atypical pneumonia Panel |
RSV1,2 | Sarbecovirus SARS-CoV-22 | KPC2 | Pneumocystis jirovecii1 |
ParaInfluenza 11,2 | SARS-CoV-2 (COVID 19) N-gene1 | OXA-232 | Mycoplasma pneumoniae1 |
ParaInfluenza 21,2 | SARS-CoV-2 (COVID 19) E-gene1 | GES1,2 | Chlamydophila psittaci1 |
ParaInfluenza 31,2 | SARS-CoV-2 (COVID 19) RdRP-gene1 | VIM2 | Chlamydia pneumoniae1 |
ParaInfluenza 41,2 | MERS Coronavirus UpE1 | NDM2 | Legionella pneumophila1 |
Enterovirus1,2 | MERS Coronavirus Orf1a1 | OXA-482 | Legionella spp/pn1 |
Bocavirus1,2 | Coronavirus HKU11 | IMP1,2 | |
Parechovirus2 | Coronavirus OC431 | EHEC Panel | New Born Panel |
Metapneumovirus1,2 | Coronavirus 229E1 | STX1-EHEC1 | TREC1 |
Adenovirus1,2 | Coronavirus NL631 | STX2-EHEC1 | KREC1 |
Picornavirus1,2 | panCoronavirus1 | EAE-EHEC1 | |
Rhinovirus1 | Bordetella pertussis1 | Non-Culturable Panel | Filovirus Panel |
Bordetella parapertussis1 | T. whipplei1 | Ebola Zaire1 | |
Tropical Panel | Bacterial Meningitis Panel | Resistance Panel | |
Zika1,2 | Escherichia coli uidA1 | MCR-12 | |
Dengue1 | Listeria monocytogenes1 | Mycoplasma Macrolide1 | |
Chikungunya1 | Streptococcus pneumoniae1 | ||
Plasmodium genus1 | Neisseria meningitidis1 | ||
Streptococcus agalactiae1 | |||
Haemophilus influenzae1 | |||
Specifications
Manufacturer | Roche Diagnostics |
Condition | New |
Weight | 121 lb (55.6 kg) |
Dimensions | W x D x H: 24 in x 24 in x 21.5 in (57.4 cm x 58.8 cm x 49.7 cm) |
Power consumption | 200–240 Vac (50/60 Hz, 1500 VA) |
Reaction volumes | 5 μL–20 μL (384-well) 10 μL–100 μL (96-well) |
Temperature control | Peltier-based heating/cooling from 37°C–95°C (20° starting temperature to perform specific melting curve analyses) |
Heating rate | 96-well block: 4.4°C/s 384-well block: 4.8°C/s |
Cooling rate | 96-well block: 2.2°C/s 384-well block: 2.5°C/s |
Excitation | LightCycler® 480 LED Lamp (430-630 nm) |
Detector | Cooled monochrome CCD camera |
Filters | Excitation (nm): 440, 465, 498, 533, 618 Detection (nm): 488, 510, 580, 610, 640, 660 |
Computer | Pentium PC with Windows 7 |