Roche LightCycler 480 || Real Time PCR Benchtop System

Roche LightCycler 480 || Real Time PCR Benchtop System

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Description

Includes:

– One Roche LightCycler 480 and accessories.

The LightCycler® 480 System is a high-performance, medium- to high-throughput PCR platform for gene detection, gene expression analysis, genetic variation analysis, and array data validation. It features advanced thermal block and optical technology for excellent well-to-well homogeneity and maximized detection sensitivity. Analyze true raw data without passive reference dyes or normalization plates, following MIQE guidelines.

Key Advantages:

  • Combination of speed, accuracy, and versatility
  • Plate-based system supports mono- or multicolor applications
  • Easily customizable for changing user requirements
  • Can be integrated into automated high-throughput workflows

Features / Benefits:

The LightCycler® 480 System can perform:

  • Gene Quantification
  • Gene Detection
  • Absolute Quantification
  • Relative Quantification / Gene Expression Analysis
  • Genetic Variation Analysis
  • Melt-curve Genotyping (with HybProbe or SimpleProbe probes)
  • Endpoint Genotyping (with hydrolysis probes)
  • Gene Scanning or Methylation Analysis (with High Resolution Melting, HRM)
  • Multiple Plate Analysis (96- or 384-well plates)

Additional advantages:

  • Fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids, mutation scanning, and SNP analysis
  • Supports basic and advanced methods for gene expression and genotyping based on endpoint analysis or melting curves
  • Wide range of probe formats and fluorescent dyes
  • Compatible with white or clear multiwell plates to optimize assay format, application, and workflow


Test Menu


Gastro Parasite Panel

Giardia2

Dientamoeba fragilis2

Cryptosporidium spp2

Blastocystis2

Entamoeba histolitica2

Gastro Virus Panel

Novovirus GG12

Novovirus GG22

Sapovirus1,2

Rotavirus2

Adenovirus1,2

Astrovirus1,2

Enterovirus1

Bocavirus1,2

Coronavirus 229E1

Coronavirus NL631

panCoronavirus1

Picornavirus1,2

Rhinovirus1

Gastro Bacteria Panel

Aeromonas2

Yersinia enterocolitica2

Campylobacter2

Shigella/EIEC2

Salmonella2

Plesiomonas1

Plesiomonas1

Respiratory Influenza Panel

Influenza A2

Influenza B2

Influenza A H1 (H1N1)1,2

Influenza A H31

Influenza A H51

Influenza A H7 (H7N9)1

Influenza A H91

Emerging Pathogens Panel

Zika Virus2

Respiratory Virus Panel

RSV1,2

ParaInfluenza 11,2

ParaInfluenza 21,2

ParaInfluenza 31,2

ParaInfluenza 41,2

Enterovirus1,2

Metapneumovirus1,2

Adenovirus1,2

Picornavirus1,2

Rhinovirus1

Bordetella pertussis1

Bordetella parapertussis1

Respiratory Coronavirus Panel

Sarbecovirus SARS-CoV-22

SARS-CoV-2 (COVID 19) N-gene1

SARS-CoV-2 (COVID 19) E-gene1

SARS-CoV-2 (COVID 19) RdRP-gene1

MERS Coronavirus UpE1

MERS Coronavirus Orf1a1

Coronavirus HKU11

Coronavirus OC431

Carbapenemase Panel

KPC2

OXA-232

GES1,2

VIM2

NDM2

OXA-482

IMP1,2

Respiratory Atypical Pneumonia Panel

Pneumocystis jirovecii1

Mycoplasma pneumoniae1

Chlamydophila psittaci1

Chlamydia pneumoniae1

Legionella pneumophila1

Legionella spp/pn1

EHEC Panel

STX1-EHEC1

STX2-EHEC1

EAE-EHEC1

New Born Panel

TREC1

KREC1

Non-Culturable Panel

T. whipplei1

Bordetella pertussis1

Bordetella parapertussis1

Filovirus Panel

Ebola Zaire1

Tropical Panel

Zika1,2

Dengue1

Chikungunya1

Plasmodium genus1

Bacterial Meningitis Panel

Escherichia coli uidA1

Listeria monocytogenes1

Streptococcus pneumoniae1

Neisseria meningitidis1

Streptococcus agalactiae1

Haemophilus influenzae1

Resistance Panel

MCR-12

Mycoplasma Macrolide1

Specifications

ManufacturerRoche Diagnostics
ConditionNew
Stock Number280
Weight121 lb (55.6 kg)
DimensionsW x D x H: 24 in x 24 in x 21.5 in (57.4 cm x 58.8 cm x 49.7 cm)
Power consumption200–240 Vac (50/60 Hz, 1500 VA)
Reaction volumes5 μL–20 μL (384-well) 10 μL–100 μL (96-well)
Temperature controlPeltier-based heating/cooling from 37°C–95°C (20° starting temperature to perform specific melting curve analyses)
Heating rate96-well block: 4.4°C/s 384-well block: 4.8°C/s
Cooling rate96-well block: 2.2°C/s 384-well block: 2.5°C/s
ExcitationLightCycler® 480 LED Lamp (430-630 nm)
DetectorCooled monochrome CCD camera
FiltersExcitation (nm): 440, 465, 498, 533, 618 Detection (nm): 488, 510, 580, 610, 640, 660
ComputerPentium PC with Windows 7